Primer

Part:BBa_K3658000:Design

Designed by: Marko Susic   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-24)


Batrachochytrium dendrobatidis (Bd) ITS region RPA forward primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The selection of the right primers for our experiments were done accordingly to the following parameters : GC content = 50%, self 3' complimentarity < 3.00 and self complimentarity < 7.00


Source

We found the Bd ITS region sequence in NCBI and used PrimedRPA software to design the primer.

References